|
|
|
|
|
| ELIZONDO,ALEJANDRA; ARAYA,JULIA; RODRIGO,RAMÓN; SIGNORINI,CINZIA; SGHERRI,CRISTIANA; COMPORTI,MARIO; PONIACHIK,JAIME; VIDELA,LUIS A. |
| Our aim was to study the influence of weight loss on the fatty acid (FA) composition of liver and erythrocyte phospholipids and oxidative stress status in obese, non-alcoholic, fatty liver disease (NAFLD) patients. Seven obese NAFLD patients who underwent subtotal gastrectomy with a gastro-jejunal anastomosis in roux and Y were studied immediately and 3 months after surgery. Seven non-obese patients who underwent anti-reflux surgery constituted the control group. Serum F2-isoprostane levels were measured by GS/NICI-MS/MS and FA composition was determined by GC. At the time of surgery, controls and obese patients exhibited a hepatic polyunsaturated fatty acid (PUFA) pattern that correlated with that of erythrocytes. Three months after surgery, NAFLD... |
| Tipo: Journal article |
Palavras-chave: Polyunsaturated fatty acids; Oxidative stress; Obesity; Weight loss. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100008 |
| |
|
|
| VARELA,NELSON M; QUIÑONES,LUIS A; ORELLANA,MYRIAM; PONIACHIK,JAIME; CSENDES,ATTILA; SMOK,GLADYS; RODRIGO,RAMÓN; CÁCERES,DANTE D; VIDELA,LUIS A. |
| CYP2E1 enzyme is related to nonalcoholic steatohepatitis (NASH) due to its ability for reactive oxygen species production, which can be influenced by polymorphisms in the gene. The aim of this study was to investigate hepatic levels, activity, and polymorphisms of the CYP2E1 gene to correlate it with clinical and histological features in 48 female obese NASH patients. Subjects were divided into three groups: (i) normal; (ii) steatosis; and (iii) steatohepatitis. CYP2E1 protein level was assayed in microsomes from liver biopsies, and in vivo chlorzoxazone hydroxylation was determined by HPLC. Genomic DNA was isolated for genotype analysis through PCR. The results showed that liver CYP2E1 content was significantly higher in the steatohepatitis (45%; p=0.024)... |
| Tipo: Journal article |
Palavras-chave: Steatohepatitis; NASH; CYP2E1; Obesity; Genotype; Chlorzoxazone. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100010 |
| |
|
|
|
